Duverger-van Bogaert M, Lambotte-Vandepaer M, Mercier M, Poncelet F
Toxicol Lett. 1982 Oct;13(3-4):203-9. doi: 10.1016/0378-4274(82)90213-2.
The irreversible protein binding of [3-14C]acrylonitrile (ACN) was evaluated in the presence of microsomes, cytosolic and post-mitochondrial fractions of rat liver. Spontaneous binding was measured after incubation of ACN in the presence of the various liver fractions at 4 degrees C and 37 degrees C respectively and in a comparative study performed at 37 degrees C in the presence of S9 or bovine serum albumin (BSA). The effect of various inducers and inhibitors was investigated. A spontaneous binding resulting from a direct alkylation by ACN seemed to play an important role; however, binding resulting from the microsomal activation of ACN into reactive intermediate(s) was also detected.
在大鼠肝脏微粒体、胞质溶胶和线粒体后组分存在的情况下，评估了[3-14C]丙烯腈（ACN）的不可逆蛋白质结合。分别在4℃和37℃下，将ACN与各种肝脏组分一起孵育后，测量自发结合情况，并在37℃下存在S9或牛血清白蛋白（BSA）的情况下进行比较研究。研究了各种诱导剂和抑制剂的作用。由ACN直接烷基化导致的自发结合似乎起重要作用；然而，也检测到了ACN经微粒体激活形成反应性中间体所导致的结合。
