Konwalinka G, Geissler D, Peschel C, Tomaschek B, Odavic R, Braunsteiner H
Acta Med Austriaca. 1982;9(3):109-10.
A microagar-culture system for the in vitro study of myelopoiesis committed stem cells has been reported. Stimulation was induced by a commercially available conditioned medium obtained from culture of histiocytoma cells. The effect of different concentrations of conditioned medium and a linear relation between the seeded cells and the formed colonies has been established. The granulocytic-monocytic character of cell aggregates has been shown by morphologic studies. Further possibilities for the use of the described method have been briefly discussed.
据报道，一种用于体外研究髓系定向干细胞的微琼脂培养系统已被开发出来。刺激是由从组织细胞瘤细胞培养物中获得的市售条件培养基诱导的。已经确定了不同浓度条件培养基的作用以及接种细胞与形成的集落之间的线性关系。通过形态学研究表明了细胞聚集体的粒细胞-单核细胞特征。本文还简要讨论了使用该方法的其他可能性。
