Rarey K E, Ross M D
Acta Otolaryngol. 1982 Sep-Oct;94(3-4):307-16. doi: 10.3109/00016488209128918.
The acute (30 min) effects of the loop diuretics piretanide, ethacrynic acid, bumetanide, furosemide, and azosemide, and the chronic (8 days) effects of furosemide and bumetanide on the zonulae occludentes (tight junctions) of the perilymph-endolymph barrier in the stria vascularis and in Reissner's membrane of the basal cochlear turns were studied by freeze-fracture. Quantitative analysis of their effects indicated that the structural integrity of the barrier was modified by either as increase or a decrease in the number, depth, and density of the strands of the tight junctions of the strial marginal cells. Only azosemide appeared to modify the tight junctions between the epithelial cells of Reissner's membrane, but it had little effect on the strial junctions. The tight junctions between the basal cells of the stria appeared to be the least affected by the various loop diuretics, although piretanide appeared to increase randomly the intercellular spaces lying between the strands.
通过冷冻蚀刻技术，研究了髓袢利尿剂吡咯他尼、依他尼酸、布美他尼、呋塞米和阿佐塞米的急性（30分钟）效应，以及呋塞米和布美他尼对蜗管基底回血管纹和Reissner膜中外淋巴-内淋巴屏障紧密连接的慢性（8天）效应。对其效应的定量分析表明，屏障的结构完整性通过血管纹边缘细胞紧密连接的条索数量、深度和密度的增加或减少而改变。只有阿佐塞米似乎改变了Reissner膜上皮细胞之间的紧密连接，但对血管纹连接影响不大。血管纹基底细胞之间的紧密连接似乎受各种髓袢利尿剂的影响最小，尽管吡咯他尼似乎会随机增加条索之间的细胞间隙。
