Nalpha-(pteroyltetra (gamma-glutamyl))-lysine as a ligand for the purification of thymidylate synthetase by affinity chromatography.
作者信息
Plante L T, Gaumont Y, Kisliuk R L
出版信息
Prep Biochem. 1978;8(2-3):91-8. doi: 10.1080/00327487808069051.
PMID:714878
Abstract
Nalpha-(pteroyltetra (gamma-glutamyl))-lysine Sepharose was synthesized and shown to be a stable high capacity affinity matrix capable of bringing about the purification of Lactobacillus casei thymidylate synthetase to maximum specific activity from crude extracts in high yield. Under conditions optimal for binding of thymidylate synthetase, dihydrofolate reductase was not bound.
摘要
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引用本文的文献
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Purification of mouse liver thymidylate synthetase by affinity chromatography using 10-methyl-5,8-dideazafolate as the affinant.以10-甲基-5,8-二氮杂叶酸为亲和剂，通过亲和色谱法纯化小鼠肝脏胸苷酸合成酶。
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