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Nalpha-(pteroyltetra (gamma-glutamyl))-lysine as a ligand for the purification of thymidylate synthetase by affinity chromatography.

作者信息

Plante L T, Gaumont Y, Kisliuk R L

出版信息

Prep Biochem. 1978;8(2-3):91-8. doi: 10.1080/00327487808069051.

DOI:10.1080/00327487808069051
PMID:714878
Abstract

Nalpha-(pteroyltetra (gamma-glutamyl))-lysine Sepharose was synthesized and shown to be a stable high capacity affinity matrix capable of bringing about the purification of Lactobacillus casei thymidylate synthetase to maximum specific activity from crude extracts in high yield. Under conditions optimal for binding of thymidylate synthetase, dihydrofolate reductase was not bound.

摘要

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引用本文的文献

1
Purification of mouse liver thymidylate synthetase by affinity chromatography using 10-methyl-5,8-dideazafolate as the affinant.以10-甲基-5,8-二氮杂叶酸为亲和剂，通过亲和色谱法纯化小鼠肝脏胸苷酸合成酶。
Experientia. 1981 Feb 15;37(2):119-20. doi: 10.1007/BF01963180.
2
Thymidylate synthetase - substrate complex formation.胸苷酸合成酶 - 底物复合物的形成。
Mol Cell Biochem. 1982 Mar 5;43(1):49-57. doi: 10.1007/BF00229539.
3
Association of RNA with thymidylate synthase from methotrexate-resistant Streptococcus faecium.来自耐甲氨蝶呤的粪肠球菌的RNA与胸苷酸合成酶的关联。
Proc Natl Acad Sci U S A. 1983 Feb;80(4):916-20. doi: 10.1073/pnas.80.4.916.