Spring K R
Am J Otolaryngol. 1982 Sep-Oct;3(5):361-6. doi: 10.1016/s0196-0709(82)80011-2.
Epithelial cell volume is a sensitive indicator of the balance between solute entry into the cell and solute exit. Solute accumulation in the cell leads to cellular swelling because the water permeability of the cell membranes is high. Similarly, solute depletion leads to cellular shrinkage. The rate of volume change under a variety of experimental conditions may be utilized to study the rate and direction of solute transport by an epithelial cell. The pathways of water movement across an epithelium may also be deduced from the changes in cellular volume. A technique for the measurement of the volume of living epithelial cells is described and a number of experiments are discussed in which cellular volume determinations provided significant new information about the dynamic behavior of epithelia.
上皮细胞体积是溶质进入细胞与溶质排出之间平衡的敏感指标。溶质在细胞内蓄积会导致细胞肿胀，因为细胞膜的水通透性很高。同样，溶质减少会导致细胞收缩。在各种实验条件下的体积变化率可用于研究上皮细胞溶质转运的速率和方向。水跨上皮移动的途径也可从细胞体积的变化中推导出来。本文描述了一种测量活上皮细胞体积的技术，并讨论了一些实验，其中细胞体积测定提供了有关上皮动态行为的重要新信息。
