van de Molengraft F J, van 't Hof M A, Herman C J, Vooijs P G
Anal Quant Cytol. 1982 Sep;4(3):217-20.
Light microscopic cytologic diagnosis of pleural and peritoneal effusions--specifically, the differentiation between atypical or reactive mesothelial cells and malignant cells--remains a problem. During the past several years, various labor-intensive special techniques have been used to obtain a better understanding of the morphology of these cell types. The present study, performed in part to provide a basis for developing more advanced quantitative cytologic techniques, employed visual light microscopic cytometry using an ocular micrometer on 750 malignant and 750 atypical mesothelial cells at a magnification of 1,000X. Our results statistically confirmed the importance of diagnostic features used in routine cytologic evaluation. In addition to the previously reported differences between cellular, nuclear and nucleolar size in malignant cells and atypical mesothelial cells in effusions, the percentage of irregular nucleoli was shown to be an important differential feature, averaging 58.8% in malignant cells as compared with 6.6% in atypical mesothelial cells.
胸膜和腹腔积液的光学显微镜细胞学诊断——特别是非典型或反应性间皮细胞与恶性细胞之间的鉴别——仍然是一个难题。在过去几年中，人们使用了各种劳动强度大的特殊技术，以便更好地了解这些细胞类型的形态。本研究部分目的是为开发更先进的定量细胞学技术提供依据，采用目视光学显微镜细胞计数法，使用目镜测微计在1000倍放大倍数下对750个恶性细胞和750个非典型间皮细胞进行计数。我们的结果从统计学上证实了常规细胞学评估中所用诊断特征的重要性。除了先前报道的积液中恶性细胞和非典型间皮细胞在细胞、细胞核和核仁大小方面的差异外，不规则核仁的百分比被证明是一个重要的鉴别特征，恶性细胞中平均为58.8%，而非典型间皮细胞中为6.6%。
