Bojar H, Stuhldreier B, Becher R, Koldovsky U, Goerz G, Merk H, Staib W
Anticancer Res. 1982 Jul-Aug;2(4):245-9.
Vertical tube rotor sucrose gradient centrifugation in the presence of the protease inhibitor sodium molybdate provide to give a deeper insight into the steroid hormone receptor status of human malignant melanoma (MM) as did previous studies using dextran-coated charcoal procedure only. As compared to endocrine dependent breast cancer, the oestrogen binding capacity of MM is low. Although in some biopsies fairly high concentrations of progestin binders were detected, the sedimentation properties were not receptor typic. Androgen binding was found to be negligible. In contrast, the presence of glucocorticoid receptors is a common feature of human MM. A human MM cell line was demonstrated to contain glucocorticoid receptors only.
在蛋白酶抑制剂钼酸钠存在的情况下进行垂直管转子蔗糖梯度离心，能比以往仅使用葡聚糖包被活性炭法的研究更深入地了解人类恶性黑色素瘤（MM）的类固醇激素受体状态。与内分泌依赖性乳腺癌相比，MM的雌激素结合能力较低。尽管在一些活检样本中检测到相当高浓度的孕激素结合物，但其沉降特性并非典型受体特征。雄激素结合可忽略不计。相反，糖皮质激素受体的存在是人类MM的一个共同特征。已证明一种人类MM细胞系仅含有糖皮质激素受体。
