Ukeshima A, Ishiyama A
Arch Histol Jpn. 1982 Jul;45(3):257-61. doi: 10.1679/aohc.45.257.
Thrombocytes of early chick embryos were observed in blood smear and whole embryo preparations stained with PAS. In the blood smear preparations, thrombocytes usually had one large glycogen mass in the cytoplasm until 3 days of incubation, and it became progressively smaller with the advance of embryonal development. While, the application of PAS to whole embryo preparations was shown to be a very useful method for observation of the blood cells in embryos at early stages when their blood could not be collected. Thrombocytes were clearly identified in situ in the embryo by PAS staining even in very early stages when no blood circulation occurred. At stage 12, distribution of the thrombocytes coincided with that of blood islands, but was limited within the area opaca. Mitotic figures of the thrombocytes were frequently found in and near the blood islands. This suggests that blood islands are the proliferation sites of the thrombocytes in early chick embryos.
用PAS染色法对早期鸡胚的血小板进行了血涂片和全胚标本观察。在血涂片标本中，孵化3天前血小板的细胞质中通常有一个大的糖原团块，随着胚胎发育的推进，糖原团块逐渐变小。同时，PAS染色应用于全胚标本被证明是观察早期胚胎血细胞的一种非常有用的方法，因为此时无法采集到胚胎的血液。即使在没有血液循环的非常早期阶段，通过PAS染色也能在胚胎原位清晰地识别出血小板。在第12阶段，血小板的分布与血岛的分布一致，但仅限于暗区。在血岛及其附近经常发现血小板的有丝分裂图。这表明血岛是早期鸡胚中血小板的增殖部位。
