De Caro A, Multigner L, Vérine H
Biochem J. 1982 Sep 1;205(3):543-9. doi: 10.1042/bj2050543.
Two major proteins have been identified in sodium citrate extracts of bovine pancreatic stones from 15 glands with lithiasis. They were found to have a molecular weight of about 24 000 and were further characterized by a variety of methods, including polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate, isoelectric focusing, two-dimensional electrophoresis, immunodiffusion, immunoelectrophoresis and determination of N-terminal residues. These two immunologically and electrophoretically different proteins were definitely shown to be immunoreactive forms of anionic and cationic trypsinogens, which are normal components of pancreatic juice. However, in contrast with both secretory trypsinogens, the stone proteins displayed an important charge heterogeneity under isoelectric-focusing conditions. A possible role for both secretory trypsinogens in pancreatic lithogenesis is suggested by the reproducibility of the data. Finally, two minor proteins with a lower molecular weight (about 11 000--13 000) have also been found to be present in all extracts, but have not yet been identified.
在取自15个患结石病腺体的牛胰石柠檬酸钠提取物中，已鉴定出两种主要蛋白质。它们的分子量约为24000，并通过多种方法作了进一步表征，包括在十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳、等电聚焦、双向电泳、免疫扩散、免疫电泳以及N末端残基的测定。这两种在免疫学和电泳方面不同的蛋白质被明确证明是阴离子和阳离子胰蛋白酶原的免疫反应形式，而胰蛋白酶原是胰液的正常成分。然而，与两种分泌型胰蛋白酶原相比，结石蛋白在等电聚焦条件下表现出重要的电荷异质性。这些数据的可重复性提示了两种分泌型胰蛋白酶原在胰腺结石形成中可能发挥的作用。最后，还发现所有提取物中都存在另外两种分子量较低（约11000 - 13000）的次要蛋白质，但尚未鉴定出来。
