大鼠卵黄囊和大鼠腹腔巨噬细胞对乙烯基吡咯烷酮与乙烯胺的聚阳离子共聚物的吸附性胞饮作用。

Adsorptive pinocytosis of polycationic copolymers of vinylpyrrolidone with vinylamine by rat yolk sac and rat peritoneal macrophage.

作者信息

Pratten M K, Cable H C, Ringsdorf H, Lloyd J B

出版信息

Biochim Biophys Acta. 1982 Dec 17;719(3):424-30. doi: 10.1016/0304-4165(82)90229-x.

Abstract

Polycationic copolymers of vinylpyrrolidone and vinylamine (10:0.77) were prepared, and 125I-labelled with either Bolton-Hunter reagent or methyl 3,5-di-[125I]iodohydroxybenzimidate. The rate of pinocytic capture of the copolymer was compared with that of 125I-labelled polyvinylpyrrolidone, using rat visceral yolk sacs and rat macrophages cultured in vitro as test systems. Whereas polyvinylpyrrolidone was captured entirely by non-adsorptive pinocytosis, the cationic derivative was captured more efficiently, probably because it adsorbs to the cell surface. Copolymer of Mr 120 000 was internalized by macrophages somewhat more rapidly than copolymer of Mr 46 000, but was excluded from the yolk sac.

摘要

制备了乙烯基吡咯烷酮与乙烯胺（10:0.77）的聚阳离子共聚物，并用博尔顿-亨特试剂或3,5-二-[¹²⁵I]碘代羟基苯甲酸甲酯进行¹²⁵I标记。以大鼠内脏卵黄囊和体外培养的大鼠巨噬细胞为测试系统，比较了共聚物的胞饮摄取速率与¹²⁵I标记的聚乙烯吡咯烷酮的摄取速率。聚乙烯吡咯烷酮完全通过非吸附性胞饮作用被摄取，而阳离子衍生物摄取效率更高，可能是因为它吸附到细胞表面。分子量为120000的共聚物被巨噬细胞内化的速度比分子量为46000的共聚物稍快，但被卵黄囊排除。