[Effect of pyridoxine, riboflavin and glutamic acid on lysosomal hydrolase activity in the liver and serum of rats during traumatic stress].

The content of 5 lysosomal hydrolases was examined in the rat liver and blood serum after compression of hind limb soft tissues in the presence of a long-term intake of excess doses of pyridoxine, riboflavin and glutamic acid. It was shown that the 14-day application of the drug complexes dramatically increased the overall content of cathepsin C, arylsulfatases A and B, beta-glucuronidase and p-acetyl-beta-D-galactosaminidase and reduced the overall content of cathepsin D in the rat liver. The non-sedimented content of the enzymes did not practically differ from the control magnitudes. In the blood serum, the content of cathepsin C and B1 approximated that seen in the control, while that of arylsulfatases A and B and p-acetyl-beta-D-galactosaminidase decreased, whereas the beta-glucuronidase content was 75% higher as compared to the basic characteristics. In the presence of administering the drug complexes, severe mechanical injury entailed the lowering of the content of the majority of rat liver lysosomal hydrolases. Besides, one could observe an essential fall of the non-sedimented content of cathepsin C and arylsulfatases A and B. The blood serum demonstrated an appreciable decrease in the content of cathepsins C and B1, p-acetyl-beta-D-galactosaminidase and arylsulfatases A and B. Thus, the fall of the non-sedimented content and diminished release of lysosomal hydrolases into the systemic circulation attest to the preservation of the structural and functional integrity of the liver cell lysosomal system during severe mechanical injury in the presence of combined excess intake of pyridoxine, riboflavin and glutamic acid.