有髓与无髓海鳗神经提取物的促有丝分裂作用。

Mitogenic effect of myelinated vs unmyelinated garfish nerve extracts.

作者信息

Yachnis A T

出版信息

Brain Res. 1982 Dec 16;253(1-2):349-52. doi: 10.1016/0006-8993(82)90708-9.

Abstract

Mitogenic activities in crude extracts of unmyelinated olfactory nerves and myelinated trigeminal nerves of the garfish Lepisosteus osseus were compared. Extracts of each nerve type were added in a range of protein concentrations to serum-starved, subconfluent cultures of BALB/c 3T3 cells. At low protein concentrations (50-250 micrograms/ml) myelinated nerve extracts produced more [3H]thymidine incorporation in the cultured cells than unmyelinated nerve extracts, while at higher concentrations (500-1000 micrograms/ml), the latter caused as much DNA synthesis as the myelinated nerve extracts, surpassing them at the highest concentrations tested. The results suggest that both axonal and myelin components contribute to the growth-promoting activity in nerve tissue.

摘要

对雀鳝有髓三叉神经和无髓嗅神经粗提物中的促有丝分裂活性进行了比较。将每种神经类型的提取物以一系列蛋白质浓度添加到血清饥饿的、未铺满的BALB/c 3T3细胞培养物中。在低蛋白质浓度（50 - 250微克/毫升）时，有髓神经提取物比无髓神经提取物在培养细胞中产生更多的[³H]胸苷掺入，而在较高浓度（500 - 1000微克/毫升）时，后者引起的DNA合成与有髓神经提取物一样多，在测试的最高浓度下超过了有髓神经提取物。结果表明，轴突和髓鞘成分都对神经组织中的生长促进活性有贡献。