Schmitz R, Nikolaizik W, Izbicki J R, Dralle H, Pichlmayr R
Cancer Detect Prev. 1982;5(3):325-9.
Six different human colorectal adenocarcinomas were xenotransplanted onto six groups of female, syngeneic BALB/c nude mice, 4 weeks of age, under standardized pathogen-free conditions, and the serum CEA levels were determined by RIA. Five tumors grew positively, yielding a tumor take of nearly 90%. One tumor showed no growth macro-or microscopically. All animals were sacrificed after 12 weeks post-transplantation. Ten untreated controls showed a mean serum CEA concentration of 0.62 +/- 0.14 ng/ml. All tumor-bearing animals, including the negative tumor take group, exhibited increased CEA levels (1.0-60.0 ng/ml), which were positively correlated to tumor volume, measured in all tumor-bearing animal groups. However, no correlation was observed between patients' serum CEA levels, measured preoperatively, and CEA concentrations in nude mice. These findings suggest that further immunohistologic studies are necessary to evaluate the difference of CEA synthesis by microscopically identical original human and transplanted tumor tissue.
将六种不同的人类结肠腺癌异种移植到六组4周龄的同基因雌性BALB/c裸鼠身上，在标准化的无病原体条件下进行，并用放射免疫分析法测定血清癌胚抗原（CEA）水平。五种肿瘤生长良好，肿瘤接种成功率近90%。一种肿瘤在宏观和微观上均未生长。移植后12周处死所有动物。十只未治疗的对照组动物血清CEA平均浓度为0.62±0.14 ng/ml。所有荷瘤动物，包括肿瘤接种阴性组，CEA水平均升高（1.0 - 60.0 ng/ml），且与所有荷瘤动物组中测量的肿瘤体积呈正相关。然而，术前测量的患者血清CEA水平与裸鼠体内CEA浓度之间未观察到相关性。这些发现表明，有必要进行进一步的免疫组织学研究，以评估在显微镜下相同的原始人类肿瘤组织和移植肿瘤组织中CEA合成的差异。
