帽结合蛋白相关多肽与鸡胚肌肉细胞质核糖核蛋白颗粒的关联

Association of cap binding protein-related polypeptides with cytoplasmic RNP particles of chick embryonic muscle.

作者信息

Chakraborty D, Mukherjee A K, Sarkar S, Lee K A, Darveau A, Sonenberg N

出版信息

FEBS Lett. 1982 Nov 22;149(1):29-35. doi: 10.1016/0014-5793(82)81064-8.

PMID:7152032

Abstract

Cap binding protein (CBP)-related polypeptides were identified in different cytoplasmic RNP particles of embryonic chick muscles using monoclonal antibody to purified CBP. A single immunoreactive peptide (Mr 78 000) was present in preparations of both free mRNP particles and a novel 10 S translation inhibitory RNP particle. In contrast, proteins isolated from these particles showed two new low-Mr immunoreactive peptides (Mr 43 000 and Mr 29 000). No CBP related protein could be detected in polysomal mRNP, although an immunoreactive Mr 43 000 CBP-related protein was present in polysomes. The relevance of the association of different CBP-related polypeptides with cytoplasmic RNP particles and polysomes are discussed.

摘要

利用针对纯化的帽结合蛋白（CBP）的单克隆抗体，在胚胎鸡肌肉的不同细胞质核糖核蛋白颗粒中鉴定出了与CBP相关的多肽。在游离的mRNA核糖核蛋白颗粒和一种新型的10S翻译抑制性核糖核蛋白颗粒的制备物中都存在一种单一的免疫反应性肽（分子量78000）。相比之下，从这些颗粒中分离出的蛋白质显示出两种新的低分子量免疫反应性肽（分子量43000和分子量29000）。尽管在多核糖体中存在一种免疫反应性的分子量43000的CBP相关蛋白，但在多核糖体mRNA核糖核蛋白中未检测到CBP相关蛋白。讨论了不同CBP相关多肽与细胞质核糖核蛋白颗粒和多核糖体之间关联的相关性。