一种涉及鸡胚肌肉中与mRNA相关蛋白质的翻译控制模型。

A model of translational control involving mRNA-associated proteins in chick embryonic muscles.

作者信息

Jain S K, Roy R K, Pluskal M G, Croall D E, Guha C, Sarkar S

出版信息

Mol Biol Rep. 1979 May 31;5(1-2):79-85. doi: 10.1007/BF00777492.

Abstract

Highly purified poly(A)-containing free and polysomal mRNP particles have been isolated by chromatography of subcellular fractions of chick embryonic muscles on oligo-dT-cellulose and elution with low salt buffer at 45 degrees. The free and polysomal mRNP represent two distinct classes of macromolecules, the free particles having a more complex nucleoprotein organization than the polysomal particles. Comparison of the protein moieties of three classes of poly(A)-containing cytoplasmic mRNP -- those released from nuclei after in vitro transcription and processing (transported mRNP), the free, and polysomal mRNP -- strongly suggests that the majority of the mRNA-associated proteins are exchanged in the cytoplasm during the various functional states of mRNA. A model of translational control involving the participation of mRNA-associated proteins in chick embryonic muscles and by analogy in other differentiated eukaryotic cells is proposed.

摘要

通过对鸡胚肌肉亚细胞组分在寡聚dT-纤维素上进行层析，并在45℃用低盐缓冲液洗脱，已分离出高度纯化的含聚腺苷酸（poly(A)）的游离和多核糖体信使核糖核蛋白（mRNP）颗粒。游离和多核糖体mRNP代表两类不同的大分子，游离颗粒的核蛋白组织比多核糖体颗粒更复杂。对三类含poly(A)的细胞质mRNP（即体外转录和加工后从细胞核释放的那些（转运的mRNP）、游离的和多核糖体mRNP）的蛋白质部分进行比较，强烈表明在mRNA的各种功能状态期间，大多数与mRNA相关的蛋白质在细胞质中进行交换。提出了一个涉及mRNA相关蛋白质参与鸡胚肌肉并类推到其他分化真核细胞的翻译控制模型。