多头绒泡菌中组蛋白基因表达的调控。I. 细胞周期中的蛋白质合成。

Control of histone gene expression in Physarum polycephalum. I. Protein synthesis during the cell cycle.

作者信息

Schofield P N, Walker I O

出版信息

J Cell Sci. 1982 Oct;57:139-50. doi: 10.1242/jcs.57.1.139.

DOI:10.1242/jcs.57.1.139

Abstract

Synchronous cultures of Physarum polycephalum were pulsed with [3H]lysine hydrochloride in S and G2 phases of the cell cycle. Plasmodial extracts were separated into nuclear, ribosomal and acid-soluble post-ribosomal cytoplasmic fractions. Core histones could be detected by staining in the nuclear fractions of both S and G2 phases, but were not detected by staining in the cytoplasmic fractions. Newly synthesized histone was present in S-phase nuclei but not in S-phase cytoplasm. The specific activity of newly synthesized histone in G2-phase nuclei decreased by at least 95% compared to S phase and no newly synthesized histone was observed in G2-phase cytoplasmic fractions. Thus histone synthesis is restricted to S phase. There are no free pools of histone in the cytoplasm of Physarum in either S or G2 phases of the cell cycle.

摘要

在多头绒泡菌的同步培养物处于细胞周期的S期和G2期时，用[3H]盐酸赖氨酸进行脉冲标记。将原质团提取物分离为细胞核、核糖体和酸溶性核糖体后细胞质部分。通过染色可在S期和G2期的细胞核部分检测到核心组蛋白，但在细胞质部分染色未检测到。新合成的组蛋白存在于S期细胞核中，而不存在于S期细胞质中。与S期相比，G2期细胞核中新合成组蛋白的比活性至少降低了95%，且在G2期细胞质部分未观察到新合成的组蛋白。因此，组蛋白合成仅限于S期。在细胞周期的S期或G2期，多头绒泡菌的细胞质中均不存在组蛋白的游离库。