Oliver S P, Smith K L
J Dairy Sci. 1982 Nov;65(11):2119-24. doi: 10.3168/jds.S0022-0302(82)82470-3.
Twenty cows were used to determine effectiveness of a nonantibiotic method for control of intramammary infection during the dry period. Right mammary quarters of cows were infused with colchicine, endotoxin, or both at 1 day prior to or at the end of lactation. Left quarters were uninfused controls within group. Samples (n = 1105) of foremilk were obtained aseptically from quarters for bacteriological analysis. Quarters were sampled during the last week of lactation, early nonlactating period, prepartum, at parturition, and 1 and 2 wk postpartum. Primary pathogens isolated were esculin + streptococci, coagulase + staphylococci, and coliform bacteria. Isolation of primary pathogens was reduced 50% in right infused quarters during the 1st wk of involution as compared to left uninfused quarters. Thirteen of 80 quarters were infected at the end of lactation (6 right and 7 left), and 20 of 80 quarters (9 right and 11 left) were infected at calving. Sixteen of the 20 infections at calving were new during the dry period. Treatment reduced infection during early involution but failed to prevent new infection during the remainder of the dry period.
选用20头奶牛来确定一种非抗生素方法在干奶期控制乳房内感染的有效性。在泌乳期前1天或泌乳期末，给奶牛的右乳房象限注入秋水仙碱、内毒素或两者。同一组内，左乳房象限作为未注入的对照。从前奶中无菌采集样本（n = 1105）进行细菌学分析。在泌乳期最后一周、非泌乳早期、产前、分娩时以及产后1周和2周对乳房象限进行采样。分离出的主要病原菌为七叶苷阳性链球菌、凝固酶阳性葡萄球菌和大肠菌群。与未注入的左乳房象限相比，在 involution 的第1周，注入的右乳房象限中主要病原菌的分离率降低了50%。在泌乳期末，80个乳房象限中有13个被感染（6个右象限和7个左象限），在产犊时，80个乳房象限中有20个被感染（9个右象限和11个左象限）。产犊时的20例感染中有16例是在干奶期新发生的。治疗减少了 involution 早期的感染，但未能预防干奶期其余时间的新感染。
