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从对照和激素处理的大鼠肝细胞中快速制备代谢活性线粒体。

Rapid preparation of metabolically active mitochondria from control and hormone-treated rat liver cells.

作者信息

Vargas A M

出版信息

J Biochem Biophys Methods. 1982 Dec;7(1):1-6. doi: 10.1016/0165-022x(82)90030-6.

DOI:10.1016/0165-022x(82)90030-6
PMID:7153452
Abstract

A method is described for the rapid isolation of mitochondria from liver cells. The method involves the breakage of hepatocytes by brief sonication followed by Percoll density gradient centrifugation. Mitochondria obtained using this method are well coupled, slightly contaminated by other organelles or membrane fractions and compare favourably with mitochondria isolated from whole liver by conventional techniques.

摘要

本文描述了一种从肝细胞中快速分离线粒体的方法。该方法包括通过短暂超声处理破碎肝细胞，然后进行Percoll密度梯度离心。用这种方法获得的线粒体偶联良好，仅受到其他细胞器或膜组分的轻微污染，与通过传统技术从全肝中分离的线粒体相比具有优势。

相似文献

1
Rapid preparation of metabolically active mitochondria from control and hormone-treated rat liver cells.从对照和激素处理的大鼠肝细胞中快速制备代谢活性线粒体。
J Biochem Biophys Methods. 1982 Dec;7(1):1-6. doi: 10.1016/0165-022x(82)90030-6.
2
A procedure for the rapid preparation of mitochondria from rat liver.一种从大鼠肝脏快速制备线粒体的方法。
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3
The effects of glucagon, phenylephrine and insulin on the phosphorylation of cytoplasmic, mitochondrial and membrane-bound proteins of intact liver cells from starved rats.胰高血糖素、去氧肾上腺素和胰岛素对饥饿大鼠完整肝细胞的细胞质、线粒体及膜结合蛋白磷酸化的影响。
Biochem J. 1982 Oct 15;208(1):221-9. doi: 10.1042/bj2080221.
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Measurement of the intramitochondrial volume in hepatocytes without cell disruption and its elevation by hormones and valinomycin.在不破坏细胞的情况下测量肝细胞线粒体内的体积及其受激素和缬氨霉素的影响而增加的情况。
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Stimulation of mitochondrial functions by glucagon treatment. Evidence that effects are not artifacts of mitochondrial isolation.胰高血糖素处理对线粒体功能的刺激作用。有证据表明这些作用并非线粒体分离过程中的人为现象。
Biochem J. 1983 Mar 15;210(3):819-27. doi: 10.1042/bj2100819.
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Rapid, quantitative isolation of mitochondria from rat liver using Ficoll gradients in vertical rotors.利用垂直转子中的菲可梯度从大鼠肝脏中快速、定量分离线粒体。
Anal Biochem. 1983 Jun;131(2):453-7. doi: 10.1016/0003-2697(83)90198-7.
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Stable changes to calcium fluxes in mitochondria isolated from rat livers perfused with alpha-adrenergic agonists and with glucagon.在用α-肾上腺素能激动剂和胰高血糖素灌注的大鼠肝脏中分离出的线粒体中，钙通量发生稳定变化。
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8
The rôle of mitochondrial pyruvate transport in the stimulation by glucagon and phenylephrine of gluconeogenesis from L-lactate in isolated rat hepatocytes.线粒体丙酮酸转运在胰高血糖素和去氧肾上腺素刺激离体大鼠肝细胞由L-乳酸进行糖异生过程中的作用。
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Rat liver mitochondria prepared in mannitol media demonstrate increased mitochondrial volumes compared with mitochondria prepared in sucrose media. Relationship to the effect of glucagon on mitochondrial function.与在蔗糖培养基中制备的线粒体相比，在甘露醇培养基中制备的大鼠肝脏线粒体显示出线粒体体积增加。与胰高血糖素对线粒体功能的影响的关系。
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Rapid isolation of metabolically active mitochondria from rat brain and subregions using Percoll density gradient centrifugation.使用Percoll密度梯度离心法从大鼠脑及其亚区域快速分离代谢活跃的线粒体。
J Neurochem. 1990 Aug;55(2):698-707. doi: 10.1111/j.1471-4159.1990.tb04189.x.

引用本文的文献

1
Effects of glucagon and Ca2+ on the metabolism of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate in isolated rat hepatocytes and plasma membranes.胰高血糖素和Ca2+对离体大鼠肝细胞及质膜中磷脂酰肌醇4-磷酸和磷脂酰肌醇4,5-二磷酸代谢的影响。
Biochem J. 1987 Feb 1;241(3):835-45. doi: 10.1042/bj2410835.
2
Inhibition of mitochondrial-matrix inorganic pyrophosphatase by physiological [Ca2+], and its role in the hormonal regulation of mitochondrial matrix volume.生理浓度的[Ca2+]对线粒体基质无机焦磷酸酶的抑制作用及其在激素调节线粒体基质体积中的作用。
Biochem J. 1989 Mar 15;258(3):817-21. doi: 10.1042/bj2580817.
3
Inhibition of Ca2(+)-induced large-amplitude swelling of liver and heart mitochondria by cyclosporin is probably caused by the inhibitor binding to mitochondrial-matrix peptidyl-prolyl cis-trans isomerase and preventing it interacting with the adenine nucleotide translocase.
环孢素抑制Ca2+诱导的肝脏和心脏线粒体大幅度肿胀，可能是由于该抑制剂与线粒体基质肽基脯氨酰顺反异构酶结合，阻止其与腺嘌呤核苷酸转位酶相互作用所致。
Biochem J. 1990 May 15;268(1):153-60. doi: 10.1042/bj2680153.