Tolson N D, Boothroyd B
J Microsc. 1982 Dec;128(Pt 3):301-5. doi: 10.1111/j.1365-2818.1982.tb04632.x.
Cultures of porcine granulosa cells have been grown on serum coated carbon films deposited on glass coverslips. Intact and detergent-treated cultures were critical point dried, chromium shadowed and removed from the coverslips by dilute hydrofluoric acid. Whole cell mounts were examined by conventional transmission electron microscopy. The advantage of this system over other methods includes the ability to: (1) examine cells with a highly rounded morphology, (2) use high cell densities, (3) study cell projections extending onto the substratum, (4) maintain cultures for long periods, (5) examine large samples easily, (6) readily use the same cultures for transmitted light and fluorescent microscopy, scanning and transmission electron microscopy (SEM and TEM).
猪颗粒细胞培养物已在沉积于玻璃盖玻片上的血清包被碳膜上生长。完整的和经去污剂处理的培养物经临界点干燥、铬蒸镀，然后用稀氢氟酸从盖玻片上取下。通过传统透射电子显微镜检查全细胞装片。该系统相对于其他方法的优点包括能够：(1) 检查具有高度圆形形态的细胞，(2) 使用高细胞密度，(3) 研究延伸到基质上的细胞突起，(4) 长时间维持培养物，(5) 轻松检查大样本，(6) 易于将相同的培养物用于透射光和荧光显微镜检查、扫描和透射电子显微镜检查（SEM 和 TEM）。
