Long M W, Williams N
Leuk Res. 1982;6(5):721-8. doi: 10.1016/0145-2126(82)90089-3.
Murine splenic megakaryocytopoiesis has been analysed and compared to that of the bone marrow. Quantification of megakaryocytes by acetylcholinesterase staining indicated a reduction in the total numbers of megakaryocytes in the spleen, with the largest decrease being in the total numbers of immature megakaryocytes. On a per organ basis, the spleen also contained a lower number of the megakaryocyte progenitor cells (CFU-Mk) than the bone marrow. The splenic and bone marrow progenitor cells had similar in vitro responses to megakaryocyte colony-stimulating activities. However, the splenic progenitor cells developed a lower number of megakaryocytes per colony, compared to bone marrow. This lower number of cell divisions was not compensated by increased endomitotic activity, since the splenic colony megakaryocytes had a similar distribution of DNA to those derived from marrow megakaryocytes. Cell cycle analysis indicated that, in contrast to marrow cells, splenic megakaryocyte progenitor cells are a rapidly-cycling population. This change in cell cycle status, together with altered proportions of progenitor cells, immature and mature megakaryocytes, suggests that the regulation and kinetics of megakaryocyte development are different in spleen and bone marrow.
对小鼠脾脏巨核细胞生成进行了分析，并与骨髓中的情况进行了比较。通过乙酰胆碱酯酶染色对巨核细胞进行定量分析表明，脾脏中巨核细胞的总数减少，其中未成熟巨核细胞的总数减少最为明显。以每个器官为基础，脾脏中巨核细胞祖细胞（CFU-Mk）的数量也比骨髓中的少。脾脏和骨髓中的祖细胞对巨核细胞集落刺激活性的体外反应相似。然而，与骨髓相比，脾脏祖细胞每个集落产生的巨核细胞数量较少。细胞分裂次数减少并未因核内有丝分裂活性增加而得到补偿，因为脾脏集落中的巨核细胞与骨髓来源的巨核细胞具有相似的DNA分布。细胞周期分析表明，与骨髓细胞不同，脾脏巨核细胞祖细胞是一个快速循环的群体。细胞周期状态的这种变化，以及祖细胞、未成熟和成熟巨核细胞比例的改变，表明脾脏和骨髓中巨核细胞发育的调控和动力学是不同的。
