Proll M A, Morgan W W
Neuropharmacology. 1982 Dec;21(12):1251-5. doi: 10.1016/0028-3908(82)90129-0.
The characteristics of accumulation of gamma-aminobutyric acid (GABA) in the retina after an intravitreal injection of the GABA-T inhibitor, gabaculine, have been studied in rats. The accumulation of GABA after injection of gabaculine was both dosage- and time-dependent. In both dark- and light-adapted-retinas. GABA continued to increase linearly for at least 90 min after an injection of gabaculine and this increase (3-fold) was similar in dark- and light-adapted rats. The GABA agonist, muscimol, and the GABA antagonist, bicuculline methiodide, significantly inhibited and facilitated, respectively, the gabaculine-induced increase in GABA levels. Previous studies have shown that accumulation of GABA following the irreversible inhibition of GABA-T was useful for measuring the rate of synthesis of GABA in the central nervous system, and these studies suggest that this technique may also be useful in the retina.
在大鼠中研究了玻璃体内注射γ-氨基丁酸转氨酶（GABA-T）抑制剂加巴库林后视网膜中γ-氨基丁酸（GABA）的积累特征。注射加巴库林后GABA的积累呈剂量和时间依赖性。在暗适应和明适应的视网膜中，注射加巴库林后GABA至少在90分钟内持续线性增加，并且这种增加（3倍）在暗适应和明适应的大鼠中相似。GABA激动剂蝇蕈醇和GABA拮抗剂甲碘化荷包牡丹碱分别显著抑制和促进了加巴库林诱导的GABA水平升高。先前的研究表明，GABA-T不可逆抑制后GABA的积累可用于测量中枢神经系统中GABA的合成速率，并且这些研究表明该技术在视网膜中可能也有用。
