Witt M, Słomski R, Kurpisz M
Acta Biochim Pol. 1982;29(3-4):311-9.
Microsomal RNA from SL2 cells was fractionated on oligo(dT)cellulose into three fractions: I was very similar to the non-fractionated RNA, II contained 28S and 18S rRNA, III [poly(A)RNA] of 4 - 50S, constituted 1.0-2.1% of the total RNA pool and was still contaminated with rRNAs. The last fraction was synthesized at the highest rate. Anti-H-2d serum precipitated cell-free translation products of 17S (heavy chain) and 10S (beta 2-microglobulin) mRNA fractions obtained by centrifugation on 10-30% linear sucrose gradient. beta 2-Microglobulin synthesis was 5-fold higher as compared with the heavy chain synthesis. Anti-H-2d serum precipitated translation products with Mr of 47 000-48 000 (H-2Dd and H-2Kd heavy chains) and 12 000 (beta 2-microglobulin). With liver mRNA, the patterns of translation products were identical, but beta 2-microglobulin synthesis was lower by a half than heavy chain synthesis. H-2d heavy chain messenger constituted 0.3-0.4% of the enriched fraction and was purified 15-20--fold.
将SL2细胞的微粒体RNA在寡聚（dT）纤维素上分级分离成三个级分：级分I与未分级的RNA非常相似，级分II含有28S和18S rRNA，级分III [聚（A）RNA]为4 - 50S，占总RNA库的1.0 - 2.1%，仍被rRNA污染。最后一个级分的合成速率最高。抗H - 2d血清沉淀了通过在10 - 30%线性蔗糖梯度上离心获得的17S（重链）和10S（β2-微球蛋白）mRNA级分的无细胞翻译产物。与重链合成相比，β2-微球蛋白的合成高5倍。抗H - 2d血清沉淀了分子量为47 000 - 48 000（H - 2Dd和H - 2Kd重链）和12 000（β2-微球蛋白）的翻译产物。对于肝脏mRNA，翻译产物的模式相同，但β2-微球蛋白的合成比重链合成低一半。H - 2d重链信使RNA占富集级分的0.3 - 0.4%，纯化了15 - 20倍。
