Zager R A
Am J Kidney Dis. 1982 Jan;1(4):227-31. doi: 10.1016/s0272-6386(82)80058-9.
The purpose of this study was to ascertain whether selected components of the uremic milieu adversely affected glomerular filtration rate (GFR), the glomerular protein filtration barrier, or the integrity of the proximal renal tubular brush border membrane. To achieve these goals, GFR and the excretion rates of albumin and of brush border derived-renal tubular epithelial antigens (RTE) were measured in normal rats and in rats with experimental nephropathies before and after the intravenous infusion of concentrated urine. This experimental protocol uniformly produced severe biochemical manifestations of uremia (for example 10-50-fold increases in BUN and creatinine, hyperphosphatemia, hyperkalemia, metabolic acidosis). However, despite these perturbations, GFR, albuminuria, and RTE excretion remained constant. To assess the influence of uremic hormonal derangements on renal function, GFR, albuminuria, and RTE excretion were measured in normal rats before and after inducing acute serum elevations of seven hormones whose concentrations are known to be increased in uremia (parathyroid hormone, growth hormone, insulin, glucagon, gastrin, prolactin, gastric inhibitory peptide). Again, GFR, albuminuria, and RTE excretion were not adversely affected. These results suggest that glomerular capillary function and proximal tubular brush border membranes are acutely resistant to many of the solute and hormonal derangements which are characteristic of uremia.
本研究的目的是确定尿毒症环境中的特定成分是否会对肾小球滤过率（GFR）、肾小球蛋白质滤过屏障或近端肾小管刷状缘膜的完整性产生不利影响。为实现这些目标，在正常大鼠和患有实验性肾病的大鼠静脉输注浓缩尿液前后，测量了GFR以及白蛋白和刷状缘来源的肾小管上皮抗原（RTE）的排泄率。该实验方案一致地产生了严重的尿毒症生化表现（例如，血尿素氮和肌酐增加10 - 50倍、高磷血症、高钾血症、代谢性酸中毒）。然而，尽管存在这些干扰，GFR、蛋白尿和RTE排泄仍保持恒定。为评估尿毒症激素紊乱对肾功能的影响，在正常大鼠中，在诱导七种已知在尿毒症中浓度会升高的激素（甲状旁腺激素、生长激素、胰岛素、胰高血糖素、胃泌素、催乳素、胃抑制肽）的血清浓度急性升高前后，测量了GFR、蛋白尿和RTE排泄。同样，GFR、蛋白尿和RTE排泄未受到不利影响。这些结果表明，肾小球毛细血管功能和近端肾小管刷状缘膜对许多尿毒症特有的溶质和激素紊乱具有急性抗性。
