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通过全细胞水解产物的纸层析法快速区分诺卡氏菌和链霉菌

RAPID DIFFERENTIATION BETWEEN NOCARDIA AND STREPTOMYCES BY PAPER CHROMATOGRAPHY OF WHOLE-CELL HYDROLYSATES.

作者信息

BECKER B, LECHEVALIER M P, GORDON R E, LECHEVALIER H A

出版信息

Appl Microbiol. 1964 Sep;12(5):421-3. doi: 10.1128/am.12.5.421-423.1964.

Abstract

Whole-cell hydrolysates were prepared from 58 strains of nocardiae and streptomycetes. Strains morphologically intermediate between the two genera and morphological variants of the same strains were included. Paper chromatograms made from the whole-cell hydrolysates clearly demonstrated meso-diaminopimelic acid as a major constituent of cultures of Nocardia spp., and LL-diaminopimelic acid as a major constituent of cultures of Streptomyces spp. In cultures of ten strains of N. madurae and of three of N. pelletieri, meso-diaminopimelic acid predominated, thereby supporting the assignment of these species to the genus Nocardia.

摘要

从58株诺卡氏菌和链霉菌中制备了全细胞水解产物。其中包括形态上介于这两个属之间的菌株以及同一菌株的形态变体。由全细胞水解产物制成的纸色谱图清楚地表明,内消旋二氨基庚二酸是诺卡氏菌属培养物的主要成分,而LL-二氨基庚二酸是链霉菌属培养物的主要成分。在10株马杜拉诺卡氏菌和3株佩氏诺卡氏菌的培养物中,内消旋二氨基庚二酸占主导,从而支持将这些菌种归为诺卡氏菌属。

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本文引用的文献

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[Current trends in the classification of actinomycetes].
Ann Soc Belg Med Trop (1920). 1962 Aug 31;42:633-49.
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The type species of the genus Nocardia.诺卡氏菌属的模式种。
J Gen Microbiol. 1962 Jan;27:1-10. doi: 10.1099/00221287-27-1-1.

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