Smith H C, Berezney R
Biochemistry. 1982 Dec 21;21(26):6751-61. doi: 10.1021/bi00269a021.
A nuclear matrix-bound in vitro replicational system that utilizes alpha-polymerase and matrix-attached DNA has been isolated from regenerating rat liver and characterized for in vitro requirements. beta-Polymerase is a minor component of the matrix in vitro system. Evidence is presented for the native association of alpha-polymerase with the nuclear matrix and for the parallel induction of matrix-bound alpha-polymerase and in vivo DNA synthesis during the proliferative response following partial hepatectomy. Our data suggest that the attachment of alpha-polymerase to the matrix may be an important step in the assembly of functional replisomes. We also demonstrate prereplicative and late-replicative rises in nuclear and matrix-bound beta-polymerase activity and discuss these results in terms of possible genome screening.
一种利用α-聚合酶和附着于核基质的DNA的核基质结合体外复制系统已从再生大鼠肝脏中分离出来，并对其体外需求进行了表征。β-聚合酶是该基质体外系统的次要成分。本文提供了α-聚合酶与核基质天然结合的证据，以及在部分肝切除后的增殖反应过程中，基质结合的α-聚合酶和体内DNA合成平行诱导的证据。我们的数据表明，α-聚合酶与基质的附着可能是功能性复制体组装中的重要一步。我们还证明了核和基质结合的β-聚合酶活性在复制前和复制后期的升高，并从可能的基因组筛选角度讨论了这些结果。
