Dean M F, Olsen I, Muir H
Biochim Biophys Acta. 1982 Dec 30;721(4):441-8. doi: 10.1016/0167-4889(82)90100-8.
Human fibroblasts totally deficient in beta-glucuronidase acquired high levels of enzyme activity when co-cultured with mouse or rabbit lymphocytes. Direct cell-to-cell contact was obligatory for this process. The enzyme acquired by the fibroblasts was shown to be identical to beta-glucuronidase from donor lymphocytes by its position of elution from DEAE-cellulose, thermal stability, mobility on polyacrylamide gels and by its antigenic determinants. The enzyme extracted from deficient fibroblasts after co-culture with lymphocytes showed no evidence of any hybridisation between human and mouse or rabbit sub-units. It is concluded that during direct cell interaction, enzymically active beta-glucuronidase is transferred directly from donor lymphocytes to deficient fibroblasts by a mechanism, previously shown not to involve normal receptor mediated endocytosis.
完全缺乏β-葡萄糖醛酸酶的人成纤维细胞在与小鼠或兔淋巴细胞共培养时获得了高水平的酶活性。此过程需要细胞间的直接接触。通过从DEAE-纤维素上洗脱的位置、热稳定性、在聚丙烯酰胺凝胶上的迁移率及其抗原决定簇，证明成纤维细胞获得的酶与供体淋巴细胞的β-葡萄糖醛酸酶相同。与淋巴细胞共培养后从缺陷成纤维细胞中提取的酶没有显示出人和小鼠或兔亚基之间任何杂交的迹象。得出的结论是，在直接细胞相互作用过程中，具有酶活性的β-葡萄糖醛酸酶通过一种先前证明不涉及正常受体介导的内吞作用的机制直接从供体淋巴细胞转移到缺陷成纤维细胞。
