Noguchi C T, Torchia D A, Schechter A N
Blood Cells. 1982;8(2):225-35.
Using 13C nuclear magnetic resonance techniques, we have studied the polymerization of hemoglobin S and intracellular polymerization in SS and AS erythrocytes. For SS erythrocytes, polymer could be detected at high oxygen saturations (greater than 90%) and increased with decreasing oxygen saturation to 70% of the total hemoglobin at complete deoxygenation. These results are consistent with the non-ideal behavior of hemoglobin S at the high intracellular concentration of 34 g/dl. The amount of polymer in AS erythrocytes was also measured as a function of oxygen saturation and is in agreement with the amount of polymer determined for homolysates using ultracentrifuge sedimentation. In general, these results on intracellular gelation are in good agreement with results predicted by cell-free studies extrapolated to the high intracellular hemoglobin concentration (34 g/dl). The significance of the detection of polymer at high oxygen saturation of SS erythrocytes is discussed in terms of morphologic deformation and erythrocyte transit through the circulation. We propose that intracellular polymerization is important in abnormal cell rheology and manifestation of disease.
利用碳-13核磁共振技术，我们研究了血红蛋白S的聚合作用以及SS型和AS型红细胞中的细胞内聚合现象。对于SS型红细胞，在高氧饱和度（大于90%）时可检测到聚合物，并且随着氧饱和度降低至完全脱氧时总血红蛋白的70%，聚合物含量增加。这些结果与血红蛋白S在细胞内34 g/dl的高浓度下的非理想行为一致。还测定了AS型红细胞中聚合物的含量与氧饱和度的函数关系，其结果与使用超速离心沉降法测定的同型裂解物中的聚合物含量相符。总体而言，这些关于细胞内凝胶化的结果与通过无细胞研究外推至细胞内高血红蛋白浓度（34 g/dl）所预测的结果高度一致。从形态变形和红细胞在循环中的转运角度讨论了在SS型红细胞高氧饱和度下检测到聚合物的意义。我们认为细胞内聚合作用在异常细胞流变学和疾病表现中具有重要作用。
