Koivula T, Koivusalo M
Prog Clin Biol Res. 1982;114:137-46.
The induction of rat liver cytoplasmic aldehyde dehydrogenase by some xenobiotics and phenobarbital are compared. An acute and a chronic treatment with polycyclic hydrocarbons and with TCDD produce a similar pattern of induced enzymes in isoelectric focusing. The result is clearly different from induction with phenobarbital in the genetically selected rat strain. Another main activity in cytoplasm consists of three different isoenzyme patterns which evidently are normal enzyme types. The induced enzyme of liver cytoplasm after chronic AAF-treatment was purified to homogeneity. The enzyme had a molecular weight of 90000 and a subunit molecular weight of 45000. It had Km-values in the millimolar range for aliphatic aldehydes and in the micromolar range for aromatic aldehydes. Both NAD and NADP were coenzymes of the purified aldehyde dehydrogenase.
比较了一些异生物质和苯巴比妥对大鼠肝脏细胞质醛脱氢酶的诱导作用。用多环烃和TCDD进行急性和慢性处理，在等电聚焦中产生相似的诱导酶模式。该结果与在基因选择的大鼠品系中用苯巴比妥诱导的结果明显不同。细胞质中的另一种主要活性由三种不同的同工酶模式组成，显然它们是正常的酶类型。慢性AAF处理后肝脏细胞质中的诱导酶被纯化至同质。该酶的分子量为90000，亚基分子量为45000。它对脂肪族醛的Km值在毫摩尔范围内，对芳香族醛的Km值在微摩尔范围内。NAD和NADP都是纯化的醛脱氢酶的辅酶。
