Molnár J, Gazdag M, Szepesi G
Pharmazie. 1982 Dec;37(12):836-8.
A new quantitative TLC method was developed for the determination of ethinylestradiol in tablets. The possible adsorption of ethinylestradiol on the starch can be avoided by enzymatic degradation, which enabled the easy extraction of the active ingredients from the tablet powder. The quantitative evaluation of the separated compounds was performed by densitometry at a wavelength of 280 nm. The relative standard deviation of the method was found to be better than +/- 2.6% in every case except Ambosex tablet where this value was +/- 4.7%. On the basis of the results obtained the method can be proposed for the routine analysis of tablets containing small amount of ethinylestradiol.
开发了一种新的薄层色谱定量方法用于测定片剂中的炔雌醇。通过酶降解可避免炔雌醇在淀粉上的可能吸附，这使得能从片剂粉末中轻松提取活性成分。分离出的化合物通过在280nm波长下的光密度测定法进行定量评估。除了安博塞克斯片剂（该值为±4.7%）外，该方法的相对标准偏差在每种情况下均优于±2.6%。基于所获得的结果，可提出该方法用于常规分析含有少量炔雌醇的片剂。
