Baricos W H, Wizer B, Muhiudeen I A, Steele R H
Res Commun Chem Pathol Pharmacol. 1982 Nov;38(2):339-42.
Addition of KCN or H2O2 to normal human urine resulted in chemiluminescence (CL). The CL increased with increasing concentrations of KCN and H2O2 and with increasing pH of the reaction mixture. KCN-induced UCL was markedly inhibited by exogenously added ascorbic acid (AA) and to varying degrees by glucose, albumin, tryptophan, hemoglobin and histidine. In contrast H2O2-induced UCL was significantly inhibited only by tryptophan. Mannitol and ethanol (hydroxyl radical scavengers) had little effect on either KCN-or H2O2-induced UCL. The KCN-sensitive and the H2O2-sensitive components were eluted in different positions from a Sephadex G15 column indicating chemically distinct compounds with molecular weights of less than 1500 daltons. We conclude that the UCL produced by H2O2 and KCN arise via interactions with chemically distinct compounds present in the urine.
向正常人尿液中添加氰化钾（KCN）或过氧化氢（H2O2）会产生化学发光（CL）。化学发光随着KCN和H2O2浓度的增加以及反应混合物pH值的升高而增强。外源性添加的抗坏血酸（AA）可显著抑制KCN诱导的超微弱化学发光（UCL），葡萄糖、白蛋白、色氨酸、血红蛋白和组氨酸也能不同程度地抑制该反应。相比之下，H2O2诱导的UCL仅被色氨酸显著抑制。甘露醇和乙醇（羟基自由基清除剂）对KCN或H2O2诱导的UCL影响很小。KCN敏感成分和H2O2敏感成分在葡聚糖G15柱上的洗脱位置不同，表明它们是分子量小于1500道尔顿的化学性质不同的化合物。我们得出结论，H2O2和KCN产生的超微弱化学发光是通过与尿液中化学性质不同的化合物相互作用而产生的。
