Raedsch R, Stiehl A, Waldherr R, Mall G, Gmelin K, Götz R, Walker S, Czygan P, Kommerell B
Z Gastroenterol. 1982 Dec;20(12):738-43.
Until now the determination of fibrotic processes in liver disease was restricted to histological examination of liver tissue. Recently a RIA was developed to determine the procollagen-type III-peptide concentrations in biological fluids. We used this RIA to measure the serum procollagen-type III-peptide concentrations in patients with chronic liver disease. Additionally in 24 patients with chronic persistent and chronic active hepatitis the collagen content in liver biopsies was determined histomorphometrically. The serum procollagen-type III-peptide concentrations in healthy controls (n = 40) were 6.4 +/- 0.6 ng/ml, in chronic persistent hepatitis (n = 47) 8.7 +/- 0.5 ng/ml, in chronic active hepatitis (n = 53) 20.8 +/- 2.9 ng/ml, and in alcoholic cirrhosis of the liver (n = 22) 47.7 +/- 6.1 ng/ml. Thus the patients with chronic active hepatitis and cirrhosis of the liver showed significantly elevated serum procollagen-type III-peptide levels. In chronic hepatitis a highly significant correlation (p less than 0.001) could be demonstrated between collagen content in liver tissue and serum procollagen-type III-peptide concentrations. The determination of serum procollagen-type III-peptide concentrations may prove a new useful parameter in biochemical evaluation of liver disease.
迄今为止，肝脏疾病中纤维化过程的测定仅限于肝组织的组织学检查。最近开发了一种放射免疫分析法（RIA）来测定生物体液中III型前胶原肽的浓度。我们使用这种RIA来测量慢性肝病患者血清中III型前胶原肽的浓度。此外，对24例慢性持续性肝炎和慢性活动性肝炎患者的肝活检组织中的胶原含量进行了组织形态计量学测定。健康对照组（n = 40）血清III型前胶原肽浓度为6.4±0.6 ng/ml，慢性持续性肝炎患者（n = 47）为8.7±0.5 ng/ml，慢性活动性肝炎患者（n = 53）为20.8±2.9 ng/ml，酒精性肝硬化患者（n = 22）为47.7±6.1 ng/ml。因此，慢性活动性肝炎和肝硬化患者血清III型前胶原肽水平显著升高。在慢性肝炎中，肝组织中的胶原含量与血清III型前胶原肽浓度之间存在高度显著的相关性（p < 0.001）。血清III型前胶原肽浓度的测定可能成为肝病生化评估中的一个新的有用参数。
