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猪丹毒杆菌稳定L型细胞及其伴随伪像的超微结构特征

Ultrastructural characterization of stable L-form cells from Erysipelothrix rhusiopathiae and of accompanying artifacts.

作者信息

Gumpert J, Todorov T, Toshkov A

出版信息

Z Allg Mikrobiol. 1978;18(6):409-14.

PMID:716473
Abstract

The stable L-form of Erysipelothrix rhusiopathiae is a typical protoplast type L-form. Cells are surrounded by a trilamellar cytoplasmic membrane only. They grow in form of aggregations in liquid media and their diameters vary between 0.1 and 2 micrometer. Always a large portion of cells undergoes lysis. It seems to be characteristic for L-form cultures of E. rhusiopathiae that always many artifact structures are formed. The artifacts are spherical particles with diameters of 0.1 micrometer to more than 3 micrometer. They can be differentiated from L-form cells only by electron microscopy. The artifacts consist of electron dense amorphous material and their surface is irregular without a clear boundary line. Obviously, these artifacts are produced from protein components of the medium and from cytoplasmatic components of the lysing L-form cells.

摘要

猪丹毒杆菌的稳定L型是典型的原生质体类型L型。细胞仅被三层细胞质膜包围。它们在液体培养基中以聚集体形式生长,直径在0.1至2微米之间。总是有很大一部分细胞发生裂解。猪丹毒杆菌L型培养物的一个特征似乎是总是形成许多人为结构。这些人为结构是直径为0.1微米至超过3微米的球形颗粒。只有通过电子显微镜才能将它们与L型细胞区分开来。这些人为结构由电子致密的无定形物质组成,其表面不规则,没有清晰的边界线。显然,这些人为结构是由培养基的蛋白质成分和裂解的L型细胞的细胞质成分产生的。

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Ultrastructural characterization of stable L-form cells from Erysipelothrix rhusiopathiae and of accompanying artifacts.猪丹毒杆菌稳定L型细胞及其伴随伪像的超微结构特征
Z Allg Mikrobiol. 1978;18(6):409-14.
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