Wurster U, Riese K, Hoffmann K
Acta Ophthalmol (Copenh). 1982 Oct;60(5):729-41. doi: 10.1111/j.1755-3768.1982.tb06733.x.
An attempt was made to establish normal values for the total protein concentrations and the enzyme activities of LDH, MDH and PGI in the intraocular fluids of rats, guinea pigs, rabbits, cats, dogs, sheep, cattle, pigs, horses and humans. Remarkably little species differences were noted in 9 of the 10 mammals with vitreal enzyme activities falling into a narrow range between 8.4 U/l (PGI, horse) and 92.4 U/l (MDH, guinea pig). All species obeyed the sequence aqueous less than vitreous less than serum with exception of the rat, where vitreous activities surpassed serum at least two-fold. The very low enzyme activities in the aqueous and vitreous humors demand special precautions against contaminations which can either derive from post-mortem storage or inadequate sampling procedures. Conversely elevated enzyme activities could be used as probes for pathological processes or as a check for a clean preparation.
我们试图确定大鼠、豚鼠、兔、猫、狗、绵羊、牛、猪、马和人类眼内液中总蛋白浓度以及乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)和磷酸葡萄糖异构酶(PGI)的酶活性的正常值。在10种哺乳动物中的9种中,玻璃体液酶活性的物种差异非常小,范围在8.4 U/l(PGI,马)至92.4 U/l(MDH,豚鼠)之间。除大鼠外,所有物种的房水、玻璃体液和血清中的酶活性顺序均为房水<玻璃体液<血清,在大鼠中玻璃体液活性至少比血清高两倍。房水和玻璃体液中极低的酶活性需要特别注意防止污染,污染可能源于死后储存或采样程序不当。相反,升高的酶活性可作为病理过程的指标或用于检查制备是否纯净。