Gertz C
Z Lebensm Unters Forsch. 1978 Oct 30;167(4):233-7. doi: 10.1007/BF01135593.
A method for the isolation and the quantitative determination of benzo(a)pyrene in food is described. The material is suspended in petroleum-ether. Benzo(a)pyrene is extracted from the organic phase as a water-soluble caffeine-complex. After re-extracting and purifying by column chromatography on silica gel the quantitative evaluation of benzo(a)pyrene is carried out by thinlayer chromatography and fluorimetric scanning of the cellulose acetate plates. To test the method benzo(a)pyrene was added to several products in concentrations of 100 to 1000 ng. A recovery of about 80% was achieved. Each analysis takes a total of 5 h.
本文描述了一种食品中苯并(a)芘的分离和定量测定方法。将样品悬浮于石油醚中，苯并(a)芘作为水溶性咖啡因络合物从有机相中萃取出来。经硅胶柱色谱再萃取和纯化后，通过薄层色谱法和醋酸纤维素板的荧光扫描对苯并(a)芘进行定量评估。为测试该方法，向几种产品中添加了浓度为100至1000 ng的苯并(a)芘，回收率约为80%。每次分析总共需要5小时。
