Stockhausen K, Bögl W, Weise H P
Z Lebensm Unters Forsch. 1978 Oct 30;167(4):256-61. doi: 10.1007/BF01135599.
A spectrophotometric technique for the identification of irradiated chicken (gallus domesticus) and doses ranging between 0.1 and 2.5 Mrad is described. Spectrophotometric determination of the sulfhydryl content in the animal tissue before (control) and after using 6,6'-Dithiodinicotinic acid is applied. Ionizing radiation causes a permant loss of sulfhydryl groups in the animal tissue such as chicken. Irradiation at room temperature and storing at -18 degrees C over a period of approximately one month does not show restitution or repair of the sulfhydryl loss. The dose-effect relation for this radiation-induced decrease of the sulfhydryl content can be described best by a double exponential function. Irradiation conditions, preparation of protein suspension from chicken, spectrometric investigations and other experimental details are reported. The results of all will be discussed in detail.
描述了一种用于鉴定辐照鸡肉（家鸡）以及剂量范围在0.1至2.5兆拉德之间的分光光度技术。应用分光光度法测定使用6,6'-二硫代烟酸前后动物组织中的巯基含量。电离辐射会导致动物组织（如鸡肉）中巯基的永久性损失。在室温下辐照并在-18℃下储存约一个月，未显示巯基损失的恢复或修复。这种辐射诱导的巯基含量降低的剂量效应关系可以用双指数函数来最好地描述。报告了辐照条件、从鸡肉制备蛋白质悬浮液、光谱研究及其他实验细节。所有结果将进行详细讨论。
