Kronborg I J, Frazer I H, Mackay I R
J Clin Lab Immunol. 1982 Dec;9(3):207-11.
A radioimmunoassay using a single cell suspension of glutaraldehyde-treated monkey hepatocytes was developed to measure antibody in human serum to liver membrane antigens. Hepatocytes were exposed to doubling dilutions of test sera in microtitre trays and binding of serum IgG was determined by exposure of the washed cells to 125I-labelled staphylococcal protein A. Specificity of binding was established according to several criteria including use of non-hepatic cells such as monkey kidney cells and various cell lines, IgG depletion of serum, absorption of binding activity by pre-exposure of serum to liver cells, and demonstration that binding was mediated by the F(ab) rather than Fc portions of immunoglobulin molecules. This radioimmunoassay distinguished autoimmune-type chronic active hepatitis (CAH) from other categories of liver disease. It should prove useful for diagnosis and classification of cases of CAH, and could facilitate identification of a liver-specific membrane antigen relevant to pathogenesis of autoimmune hepatitis.
开发了一种使用戊二醛处理的猴肝细胞单细胞悬液的放射免疫测定法，以测量人血清中针对肝膜抗原的抗体。将肝细胞暴露于微量滴定板中系列稀释的测试血清中，并通过将洗涤后的细胞暴露于125I标记的葡萄球菌蛋白A来测定血清IgG的结合情况。根据几个标准确定结合的特异性，包括使用非肝细胞，如猴肾细胞和各种细胞系，血清IgG的去除，血清预先暴露于肝细胞对结合活性的吸收，以及证明结合是由免疫球蛋白分子的F(ab)而非Fc部分介导的。这种放射免疫测定法可将自身免疫型慢性活动性肝炎（CAH）与其他类型的肝病区分开来。它应被证明对CAH病例的诊断和分类有用，并有助于鉴定与自身免疫性肝炎发病机制相关的肝特异性膜抗原。
