人内皮细胞培养物对不同类型和处理的人血清、培养处理以及不同氧浓度的增殖反应。

Proliferative response of human endothelial cultures to various types and treatments of human sera, to culture treatments and to various oxygen concentrations.

作者信息

Schoeffel U, Maenner H, Shiga J, Mittermayer C

出版信息

Pathol Res Pract. 1982 Dec;175(4):348-64. doi: 10.1016/S0344-0338(82)80048-4.

Abstract

Endothelial cells from the human umbilical vein were exposed to different human sera, differently treated serum, to various oxygen concentrations, and various culture treatments. Endothelial proliferation was determined by measuring the uptake of [3H]-thymidine by means of autoradiography and presented as thymidine labeling index (TI) values. TI values differed according to different serum concentrations, serum types, serum preparations (WBS-PDS) and serum pretreatments. Low oxygen concentrations in the incubator atmosphere induced an early DNA synthesis response without an increase in cell number. The addition of the protease inhibitors aprotinin and soybean trypsin inhibitor to the culture medium resulted in a dose dependent TI decrease, whereas PMSF showed no influence.

摘要

人脐静脉内皮细胞暴露于不同的人血清、不同处理的血清、不同的氧浓度以及不同的培养处理条件下。通过放射自显影法测量[3H] - 胸腺嘧啶核苷的摄取来测定内皮细胞增殖，并以胸腺嘧啶核苷标记指数（TI）值表示。TI值因不同的血清浓度、血清类型、血清制剂（WBS - PDS）和血清预处理而有所不同。培养箱环境中的低氧浓度诱导早期DNA合成反应，但细胞数量没有增加。向培养基中添加蛋白酶抑制剂抑肽酶和大豆胰蛋白酶抑制剂会导致TI呈剂量依赖性降低，而苯甲基磺酰氟（PMSF）则无影响。