Fried M, Gyr K, Beglinger C, Jeker L, Stalder G A, Varga L
Regul Pept. 1982 Dec;5(1):27-33. doi: 10.1016/0167-0115(82)90072-6.
In four conscious dogs with chronic gastric and pancreatic Thomas fistulas we studied the effect of 99% pure cholecystokinin-33 (CCK-33) solutions on pancreatic secretion and PP release. CCK-33 was dissolved in 0.154 M NaCl alone or in the same solution containing 1 g per 100 ml dog albumin. The response of pancreatic protein output to increasing doses of CCK-33 (0.5, 1, 2, 4 IDU/kg per h) was significantly (P less than 0.05) higher when CCK was dissolved in NaCl with albumin than in NaCl alone. These results were confirmed by measuring CCK immunoreactivity in samples from tips of infusion lines by a gastrin radioimmunoassay. Release of pancreatic polypeptide (PP) following increasing doses of CCK-33 was also significantly (P less than 0.05) elevated when CCK was dissolved in an albumin-containing solution. There was a significant (P less than 0.02) correlation between plasma concentrations of PP and pancreatic protein output. This study suggests that albumin should be added to CCK-33 solutions to preserve biological activity. The biological effect of CCK-33 may be substantially underestimated if albumin is omitted.
在四只患有慢性胃和胰腺托马斯瘘管的清醒犬中，我们研究了99%纯胆囊收缩素-33（CCK-33）溶液对胰腺分泌和胰多肽（PP）释放的影响。CCK-33单独溶解于0.154 M氯化钠中，或溶解于每100 ml含1 g犬白蛋白的相同溶液中。当CCK溶解于含白蛋白的氯化钠溶液中时，胰腺蛋白质输出对递增剂量的CCK-33（0.5、1、2、4国际单位/千克每小时）的反应显著（P小于0.05）高于单独溶解于氯化钠中时。通过胃泌素放射免疫测定法测量输液管尖端样品中的CCK免疫反应性，证实了这些结果。当CCK溶解于含白蛋白的溶液中时，递增剂量的CCK-33后胰多肽（PP）的释放也显著（P小于0.05）升高。PP的血浆浓度与胰腺蛋白质输出之间存在显著（P小于0.02）相关性。本研究表明，应向CCK-33溶液中添加白蛋白以保持生物活性。如果省略白蛋白，CCK-33的生物学效应可能会被大大低估。
