Chen S, Weller M A, Penney D G
Scan Electron Microsc. 1982(Pt 2):859-67.
Carbon monoxide (CO) is a well know constituent of cigarette smoke and automobile exhaust. The purpose of this study was to determine the effect of CO on the first line of lung defense, the alveolar macrophage (AM). Free lung cells were obtained by bronchoalveolar lavage with Dulbecco's phosphate buffered saline from rats exposed to 500 ppm CO from birth until 33 days of age and from littermate controls. Morphological and functional parameters of the exposed cells showed minor changes. Hemoglobin concentration and hematocrit were significantly elevated in the CO-exposed group. Recovery yield of lavage fluid, wet lung weight, and lung displaced volume were identical to controls. Neither number of AM, viability, maximal diameter, surface area nor acid phosphatase activity of exposed AM appeared to be different from controls. However, in a test with fluorescent latex spheres, the phagocytic ability of AM was found to be enhanced in CO-exposed rats and was correlated with an increased percentage of spread forms of AM adhering to glass coverslips. The surface features of the AM were somewhat modified. Number and percentage of granular leukocytes were statistically increased in lavages following chronic exposure to CO. These alterations contrast with the effects of diesel exhaust and cigarette smoke.
一氧化碳（CO）是香烟烟雾和汽车尾气中广为人知的成分。本研究的目的是确定CO对肺部第一道防线——肺泡巨噬细胞（AM）的影响。通过用杜尔贝科磷酸缓冲盐水对从出生到33日龄暴露于500 ppm CO的大鼠及其同窝对照进行支气管肺泡灌洗，获取游离肺细胞。暴露细胞的形态和功能参数显示出微小变化。CO暴露组的血红蛋白浓度和血细胞比容显著升高。灌洗液的回收产量、湿肺重量和肺置换体积与对照组相同。暴露的AM的数量、活力、最大直径、表面积或酸性磷酸酶活性似乎与对照组没有差异。然而，在一项使用荧光乳胶球的试验中，发现CO暴露大鼠的AM吞噬能力增强，并且与附着在玻璃盖玻片上的伸展形态AM百分比增加相关。AM的表面特征有所改变。长期暴露于CO后，灌洗液中粒细胞的数量和百分比在统计学上增加。这些变化与柴油废气和香烟烟雾的影响形成对比。
