人白细胞干扰素对培养细胞细胞质膜通透性的影响。

Effect of human leukocyte interferon on the permeability of the cytoplasma membrane of cultured cells.

作者信息

Degré M

出版信息

Acta Pathol Microbiol Scand B. 1978 Oct;86B(5):303-7. doi: 10.1111/j.1699-0463.1978.tb00048.x.

DOI:10.1111/j.1699-0463.1978.tb00048.x

Abstract

The effect of human leukocyte interferon on the cytoplasma membrane of cultured homologous cells has been investigated. U-amnion cells were labelled with (1-14C) alpha-aminoisobutyric acid (AIB), a low-molecular-weight non-metabolizable amino acid. Following uptake, the AIB is released spontaneously from the cells. Treatment of cells with interferon reduced the AIB release. This effect was dosedependent, was neutralized by anti-interferon serum and was strongly reduced by trypsin treatment.

摘要

已对人白细胞干扰素对培养的同源细胞细胞质膜的作用进行了研究。用人羊膜细胞用（1-14C）α-氨基异丁酸（AIB）进行标记，AIB是一种低分子量的不可代谢氨基酸。摄取后，AIB会自发地从细胞中释放出来。用干扰素处理细胞可减少AIB的释放。这种作用呈剂量依赖性，可被抗干扰素血清中和，并且经胰蛋白酶处理后会大大降低。