人干扰素对体外培养细胞细胞膜作用的功能及超微结构研究

Functional and ultrastructural studies of the effects of human interferon on cell membranes of in vitro cultured cells.

作者信息

Degré M, Hovig T

出版信息

Acta Pathol Microbiol Scand B. 1976 Dec;84B(6):347-58. doi: 10.1111/j.1699-0463.1976.tb01952.x.

DOI:10.1111/j.1699-0463.1976.tb01952.x

Abstract

The effect of human leukocyte interferon on cultured U-amnion cells was examined, and several biological parameters were registered. Multiplication of Vesicular stomatitis virus and the virus-produced cytopathogenic effect was prevented. The growth rate of uninfected cells was reduced, as well as the spontaneous release of 3H-uridine. These effects were observed following treatment with 10 units of interferon per ml. No morphological alterations could be detected by scanning electron microscopy after 24 or 72 hours treatment with up to 2000 units interferon per ml.

摘要

研究了人白细胞干扰素对培养的人羊膜细胞的作用，并记录了几个生物学参数。水泡性口炎病毒的增殖及该病毒产生的细胞病变效应受到抑制。未感染细胞的生长速率降低，同时3H-尿苷的自发释放也减少。在每毫升使用10单位干扰素处理后观察到了这些效应。在用每毫升高达2000单位干扰素处理24或72小时后，通过扫描电子显微镜未检测到形态学改变。