Rodríguez M J, Ortiz M, Vázquez A, Lluch M, Ponz F
Rev Esp Fisiol. 1982 Dec;38(4):397-401.
Kinetics of sugar transport inhibition by phlorizin in rat intestine has been studied in vivo. Jejunum lumen was perfused during a series of successive 1 minute periods of absorption. Total sugar absorption was assumed to be the sum of a mediated transport component plus a passive one persisting in the presence of 5 X 10(-4) M phlorizin. The values of transport rates, corrected in this way, showed Michaelis-Menten kinetics and proved that inhibition of D-glucose, D-galactose and 6-deoxy-D-glucose transport by low concentrations of phlorizin (5 X 10(-6) M) displays a typical competitive kinetics (apparent Ki = 4-10 X 10(-6) M) as had been long before reported from in vitro experiments.
已在体内研究了根皮苷对大鼠肠道糖转运的抑制动力学。在一系列连续的1分钟吸收期内对空肠腔进行灌注。总糖吸收被认为是介导转运成分与在5×10⁻⁴M根皮苷存在下持续存在的被动成分之和。以这种方式校正后的转运速率值显示出米氏动力学，并证明低浓度根皮苷（5×10⁻⁶M）对D-葡萄糖、D-半乳糖和6-脱氧-D-葡萄糖转运的抑制表现出典型的竞争性动力学（表观Ki = 4 - 10×10⁻⁶M），这与之前体外实验报道的结果一致。
