Kitikoon V, Rivera E R
Southeast Asian J Trop Med Public Health. 1982 Dec;13(4):519-30.
Nembutal, menthol, Ketaset, and Curarine were used to anesthetize beta race of Tricula aperta snails in preparation for study by scanning electron microscopy. The snails remained sensitive to the glutaraldehyde fixative in low level anesthesia of nembutal, menthol and Ketaset. Snails were also sensitive to Curarine and retracted into their shells. The best relaxations were obtained by initial anesthesia with nembutal or menthol followed by curarine. Snails relaxed by this procedure showed relatively little reaction to the fixative solution and remained extended outside their shells. The surface ultrastructure of the snails was well preserved, showing microvillate, secretory, and ciliated sensory cells. The mucoid secretions remained on the surface of cilia on the body and foot of the snails as spherical granules. There were two types of ciliated cells: (1) Those with few short cilia showing 1-3 microns above the microvilli of surrounding cells and (2) Those with numerous cilia up to 10 microns long. Results of transmission electron microscopic studies of these snails show no disruption of the ultrastructure of the surface cells.
使用戊巴比妥、薄荷醇、氯胺酮和箭毒对扁卷螺β族蜗牛进行麻醉，为扫描电子显微镜研究做准备。在戊巴比妥、薄荷醇和氯胺酮的低水平麻醉下，蜗牛对戊二醛固定剂仍保持敏感。蜗牛对箭毒也很敏感，并会缩进壳里。通过先用戊巴比妥或薄荷醇麻醉，然后使用箭毒，可获得最佳的松弛效果。通过此程序松弛的蜗牛对固定剂溶液的反应相对较小，并保持伸出壳外的状态。蜗牛的表面超微结构保存良好，显示出微绒毛状、分泌性和纤毛感觉细胞。黏液分泌物以球形颗粒的形式留在蜗牛身体和足部纤毛的表面。有两种类型的纤毛细胞：（1）那些短纤毛较少，在周围细胞的微绒毛上方伸出1 - 3微米的细胞；（2）那些有许多长达10微米纤毛的细胞。对这些蜗牛进行透射电子显微镜研究的结果表明，表面细胞的超微结构没有受到破坏。
