Akhrem A A, Andrianov V T, Bokut S B, Luka Z A, Kissel M A, Skornyakova T G, Kisselev P A
Biochim Biophys Acta. 1982 Nov 8;692(2):287-95. doi: 10.1016/0005-2736(82)90533-8.
The interaction of rat liver microsomal cytochrome P-450 with phospholipids has been investigated employing differential scanning microcalorimetry. It is shown that the thermotropic behaviour of phospholipid vesicles reconstituted with cytochrome P-450 depends on liposome composition, protein concentration and the mode of reconstituted system preparation. From the delta H dependence on protein concentration in proteoliposomes it was calculated that one cytochrome P-450 molecule influence 350 +/- 50 dimyristoylphosphatidylcholine (DMPC) molecules. The electrostatic interaction of cytochrome P-450 and negatively charged phospholipid, phosphatidylinositol (PI), mixed with DMPC involves the temperature stabilization of proteoliposomes at a phase transition of phospholipid bilayers. The thermal denaturation temperature is increased due to negatively charged PI added.
利用差示扫描量热法研究了大鼠肝脏微粒体细胞色素P-450与磷脂的相互作用。结果表明，用细胞色素P-450重构的磷脂囊泡的热致行为取决于脂质体组成、蛋白质浓度和重构系统的制备方式。根据蛋白脂质体中ΔH对蛋白质浓度的依赖性计算得出，一个细胞色素P-450分子影响350±50个二肉豆蔻酰磷脂酰胆碱（DMPC）分子。细胞色素P-450与带负电荷的磷脂磷脂酰肌醇（PI）（与DMPC混合）的静电相互作用涉及在磷脂双层的相变时蛋白脂质体的温度稳定化。由于添加了带负电荷的PI，热变性温度升高。
