Lister J L, Virgo B B
Can J Physiol Pharmacol. 1982 Oct;60(10):1247-50. doi: 10.1139/y82-182.
The basal activities of aniline hydroxylase (AH), hexobarbital hydroxylase (HH), and ethylmorphine N-demethylase (ED) were measured in the 9000 X g supernatant of kidneys and lungs from male and female rats. No ED activity was detected in any tissue although all tissues N-demethylated three other substrates. The activities of AH and HH were not sex dependent in either kidney or lung. Similarly, pulmonary and renal microsomal protein concentrations were independent of sex. In addition, cytochrome P-450 levels in the kidney were the same in males and females (pulmonary P-450 was not measured). The pulmonary hydroxylases were more active than the renal enzymes in both sexes. In males, phenobarbital (ip, 50 mg X kg-1 X day-1 for 3 days) failed to induce AH or HH in either kidney or lung; it did not increase the weight or microsomal protein levels of these organs and it also failed to increase renal P-450. Thus, the basal activities of AH and HH in lungs and kidneys are not different in male and female rats and are not increased by phenobarbital.
在雄性和雌性大鼠的肾脏和肺脏9000×g上清液中测量了苯胺羟化酶（AH）、己巴比妥羟化酶（HH）和乙基吗啡N-脱甲基酶（ED）的基础活性。尽管所有组织都能使其他三种底物发生N-脱甲基反应，但在任何组织中均未检测到ED活性。肾脏和肺脏中AH和HH的活性均不依赖于性别。同样，肺和肾微粒体蛋白浓度也与性别无关。此外，雄性和雌性大鼠肾脏中的细胞色素P-450水平相同（未测量肺中的P-450）。两性中肺羟化酶的活性均高于肾酶。在雄性大鼠中，苯巴比妥（腹腔注射，50mg·kg⁻¹·天⁻¹，共3天）未能诱导肾脏或肺中的AH或HH；它没有增加这些器官的重量或微粒体蛋白水平，也未能增加肾脏中的P-450。因此，雄性和雌性大鼠肺和肾中AH和HH的基础活性没有差异，且不受苯巴比妥的影响。
