Tateno H, Mikamo K
Cytogenet Cell Genet. 1982;33(4):333-9. doi: 10.1159/000131780.
Following testicular X-irradiation of Chinese hamster (Cricetulus griseus) males eight progeny (five males and three females) with reciprocal translocatons (heterozygotes) were obtained. They were all phenotypically normal but semi-sterile. Homozygotes were produced successfully with CHAT3 (2p-;9q+) and CHAT5 (2q-;8q+). They were proven to be fertile. Thus, two homozygous stocks with distinct marker chromosomes were established. CHAT3 homozygotes have shown a high incidence of diabetes, while CHAT5 nomozygotes had no genetic burden. Progeny tests of outcrosses and intercrosses with the heterozygotes and homozygotes were performed in both stocks. Karyological analysis of the first cleavage division showed the marker chromosomes of both stocks to be useful in distinguishing the paternal chromosome complements from the maternal ones.
对中国仓鼠（黑线仓鼠）雄性进行睾丸X射线照射后，获得了8只具有相互易位（杂合子）的后代（5只雄性和3只雌性）。它们在表型上均正常，但半不育。利用CHAT3（2号染色体短臂缺失；9号染色体长臂增加）和CHAT5（2号染色体长臂缺失；8号染色体长臂增加）成功培育出了纯合子。经证实它们可育。因此，建立了两个具有不同标记染色体的纯合品系。CHAT3纯合子显示出高糖尿病发病率，而CHAT5纯合子没有遗传负担。对两个品系的杂合子和纯合子进行了与异交和杂交的后代测试。第一次卵裂的核型分析表明，两个品系的标记染色体可用于区分父本染色体组和母本染色体组。
