Kochanowski B A, Sherman A R
Growth. 1982 Summer;46(2):126-34.
Groups of 10 pregnant rats weighing 200-215 g were fed ad libitum diets containing 10, 100, or 250 ppm iron (Fe) throughout gestation and lactation. Litters were adjusted at birth to contain 7 pups, and on day 17 of lactation, dams and pups were sacrificed. Fe status was determined, and tissues from pups were assayed for parameters of cellular growth. Fe-deficient pups (10 ppm Fe) had slightly lower body weights, lower hemoglobin, serum Fe, and liver Fe levels than those fed diets containing 100 or 250 ppm Fe. Thymus was most severely affected by Fe-deficiency. Organ weight, per cent body weight, and total DNA were decreased by at least 40% in Fe-deficient pups. Spleens from 10 ppm Fe pups had 30% less total DNA compared to controls due to decreased organ weight. There were no changes in spleen protein, lipid or DNA concentrations due to Fe deficiency. Measures of DNA and protein parameters in liver were similar among groups, although a 60% increase in liver lipid concentration was observed in pups from dams fed 10 ppm Fe. The results suggest that nutritional Fe deficiency during reproduction produces alterations in cellular growth of rapidly-divided lymphoid organs in offspring, while accumulation of DNA and protein in the liver is unaffected.
将10只体重在200 - 215克的怀孕大鼠分为几组，在整个妊娠期和哺乳期随意喂食含10、100或250 ppm铁（Fe）的日粮。出生时调整每窝幼崽数量至7只，在哺乳期第17天，处死母鼠和幼崽。测定铁状态，并检测幼崽组织的细胞生长参数。缺铁幼崽（10 ppm铁）的体重、血红蛋白、血清铁和肝脏铁水平略低于喂食含100或250 ppm铁日粮的幼崽。胸腺受缺铁影响最为严重。缺铁幼崽的器官重量、占体重百分比和总DNA至少降低40%。由于器官重量减轻，10 ppm铁幼崽的脾脏总DNA比对照组少30%。缺铁对脾脏蛋白质、脂质或DNA浓度没有影响。尽管在喂食10 ppm铁的母鼠所生幼崽中观察到肝脏脂质浓度增加了60%，但各组肝脏中DNA和蛋白质参数的测量结果相似。结果表明，繁殖期间的营养性缺铁会导致后代快速分裂的淋巴器官细胞生长发生改变，而肝脏中DNA和蛋白质的积累不受影响。
