Karlovský P, Dadák V
Folia Microbiol (Praha). 1982;27(6):460-4. doi: 10.1007/BF02876460.
The paper describes the construction of a new type of ion-selective electrode sensitive to tetraphenylborate (TPB-) and its electric characteristics. The electrode responds to increasing concentrations of the TPB- anion in accordance with the Nernst equation and can be used down to 0.1 microM concentration. The applicability of the electrode for measuring the membrane potential (positive inside) was proved in inside-out oriented membrane vesicles derived from Paracoccus denitrificans. The calculated values were 175 +/- 12 mV with NADH and 180 +/- 6 mV with succinate.
本文描述了一种对四苯硼酸盐（TPB-）敏感的新型离子选择性电极的构建及其电学特性。该电极根据能斯特方程对TPB-阴离子浓度的增加做出响应，可用于低至0.1微摩尔浓度的测量。在源自反硝化副球菌的内向外取向膜囊泡中证明了该电极用于测量膜电位（内部为正）的适用性。使用NADH时计算值为175±12 mV，使用琥珀酸时为180±6 mV。
