Udaya Bhaskar S, Nanda Kumar N V
J Assoc Off Anal Chem. 1982 Nov;65(6):1297-8.
The Michaelis-Menten constant (Km) for cholinesterases from different liver sources, the inhibitor concentration for 50% inhibition (I50), and the lower limit of estimation were determined for 3 organophosphorus pesticides and their oxygen analogs. Maximum sensitivity in organophosphorus pesticide determinations can be achieved by choosing the enzyme source which has a higher Km value. An inverse relationship is suggested between Km and the lower limit of estimation.
测定了3种有机磷农药及其氧类似物对不同肝脏来源胆碱酯酶的米氏常数（Km）、50%抑制率时的抑制剂浓度（I50）以及估计下限。通过选择具有较高Km值的酶源，可在有机磷农药测定中实现最大灵敏度。Km与估计下限之间呈负相关。
