Keenan R W, Martinez R A, Williams R F
J Biol Chem. 1982 Dec 25;257(24):14817-20.
Equilibration of the phosphorus in radioactive phosphoric acid with the phosphorus in phosphorus oxychloride occurs if these compounds are refluxed together for approximately 24 h. This observation led us to develop a method for the preparation of radioactive phosphorus oxychloride on a small scale with high specific radioactivity. The labeled phosphorus oxychloride may be utilized directly in a one-pot reaction for the preparation of labeled phosphate esters or for the synthesis of more selective phosphorylating agents such as cyanoethyl phosphate. Since the method is very simple and capable of yielding highly labeled radioactive phosphate esters on a small scale, it is applicable to a number of different problems. The preparation of 32P-labeled dolichyl phosphate is described utilizing this procedure.
如果将放射性磷酸中的磷与三氯氧磷中的磷一起回流约24小时，它们之间会发生平衡。这一观察结果促使我们开发一种小规模制备高比放射性三氯氧磷的方法。标记的三氯氧磷可直接用于一锅法反应，以制备标记的磷酸酯或合成更具选择性的磷酸化试剂，如氰乙基磷酸。由于该方法非常简单，能够小规模制备高标记的放射性磷酸酯，因此适用于许多不同的问题。利用此程序描述了32P标记的磷酸多萜醇的制备。
